论文部分内容阅读
目的:探索CCAAT/增强子结合蛋白α(C/EBPα)基因对HeLa细胞增殖和定向迁移的影响。方法:用MTT方法检测C/EBPα基因重组质粒转染组、空质粒转染组和未转染组对HeLa细胞增殖的影响。用Transwell侵袭转移模型评价He-La细胞的迁移情况。其中,不同上室中分别加入C/EBPα基因重组质粒转染组、空质粒转染组和未转染组的HeLa细胞,下室中加入含20%FBS的培养基。结果:C/EBPα基因重组质粒转染组、转染空质粒组和未转染HeLa细胞组的吸光度值分别为0.21±0.01、0.24±0.01和0.24±0.02,重组质粒转染组与空质粒转染组和未转染HeLa细胞组比较差异有统计学意义(P<0.05)。Transwell实验发现重组质粒转染组与空质粒转染组和未转染HeLa细胞组相比,细胞迁移能力减弱,细胞穿膜数分别为12.0±2.2、39.0±3.2和39.6±2.6,重组质粒转染组与空质粒转染组和未转染HeLa细胞组比较差异有统计学意义(P<0.05)。结论:C/EBPα基因能抑制子宫颈癌HeLa细胞的增殖和迁移能力。
Objective: To explore the effects of CCAAT / enhancer binding protein α (C / EBPα) gene on the proliferation and migration of HeLa cells. Methods: The effects of C / EBPα gene recombinant plasmid transfection group, empty plasmid transfection group and untransfected group on the proliferation of HeLa cells were detected by MTT assay. The Transwell invasion and metastasis model was used to evaluate He-La cell migration. Among them, C / EBPα gene recombinant plasmid transfection group, empty plasmid transfection group and untransfected HeLa cells were added in different upper chambers, and medium containing 20% FBS was added in the lower chamber. Results: The absorbance values of C / EBPα gene transfection group, transfected empty plasmid group and untransfected HeLa cell group were 0.21 ± 0.01, 0.24 ± 0.01 and 0.24 ± 0.02, respectively. The recombinant plasmids transfected with empty plasmid There was significant difference between the Hela cells and untransfected Hela cells (P <0.05). Transwell experiments showed that compared with the empty plasmid transfected group and the untransfected HeLa cell group, the migration ability of the recombinant plasmid transfected group was weakened, the cell penetrating numbers were 12.0 ± 2.2,39.0 ± 3.2 and 39.6 ± 2.6 respectively, There was significant difference between the transfected group and the untransfected HeLa cells (P <0.05). Conclusion: C / EBPα gene can inhibit the proliferation and migration of cervical cancer HeLa cells.