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目的:动脉粥样硬化是一种慢性免疫炎症性疾病,其脆性斑块的两个主要特征是含有大量的炎症免疫介质和巨噬细胞。oxHDL和oxLDL被发现存在于粥样斑块内,探索、比较其对炎症免疫介质产生的影响有助于进一步阐明As发生发展的机制。方法:用RT-PCR和流式细胞术等方法观察oxHDL3和oxLDL对THP-1单核源性巨噬细胞表达4种动脉粥样硬化相关基因(炎症免疫反应相关)IL-1β、IL-6、CD86、HLA-DR的影响。结果:THP-1单核源性巨噬细胞经oxHDL3和oxLDL处理后,IL-1β、IL-6的mRNA水平明显上调,CD86、HLA-DR的表面表达增强,CD86+、HLA-DR+细胞的百分比显著提高。而天然HDL3和LDL不具有上述效应。结论:oxHDL3和oxLDL经诱导或增强巨噬细胞表达IL-1β、IL-6、CD86和HLA-DR,可能参与动脉粥样硬化病变的形成与发展。
AIM: Atherosclerosis is a chronic immunological inflammatory disease whose two major hallmarks of fragile plaque contain a large amount of inflammatory mediators and macrophages. oxHDL and oxLDL were found in atherosclerotic plaques, to explore and compare their impact on inflammatory mediators help to further elucidate the mechanism of As development. Methods: The effects of oxHDL3 and oxLDL on the expression of four atherosclerosis related genes (IL-1β and IL-6) in THP-1 monocyte-derived macrophages were observed by RT-PCR and flow cytometry , CD86, HLA-DR. Results: The mRNA levels of IL-1β and IL-6 in THP-1 monocyte-derived macrophages treated with oxHDL3 and oxLDL were significantly up-regulated, and the expression of CD86 and HLA-DR was enhanced. The percentages of CD86 + and HLA- Significantly increased. While natural HDL3 and LDL do not have the above effects. CONCLUSION: OxHDL3 and oxLDL may be involved in the formation and development of atherosclerotic lesions by inducing or enhancing the expression of IL-1β, IL-6, CD86 and HLA-DR in macrophages.