目的：探讨脂质体／ＨＳＶ—ＴＫ重组 ＤＮＡ对膀胱癌细胞的转导和在细胞内的活性表达。方法：用脂质体／ＤＮＡ复合物将 ＨＳＶ－ＴＫ基因逆转录病毒重组子（ｐＬＸＳＮ－ＴＫ）导入膀胱癌细胞株 ＢＩＵ８７。携带 ＨＳＶ一ＴＫ基因的 ＢＩＵ８７细胞（ＢＩＵ８７—ＴＫ）由 Ｇ４１８筛选获得。经过 Ｓｏｕｔｈｅｒｎ杂交和细胞原位杂交检测后，观测 ＡＣＶ对 ＢＩＵ８７－ＴＫ细胞存活的影响。结果： Ｓｏｕｔｈｅｒｎ印迹杂交证实 ＨＳＶ—ＴＫ基因存在于 ＢＩＵ８７－ＴＫ细胞染色体中。细胞原位杂交检测 ＨＳＶ－ＴＫｍＲＮＡ的表达。外源ＤＮＡ的导入以及ＨＳＶ－ＴＫ和Ｎｅｏ基因表达不会改变细胞生长特性。然而，在ＡＣＶ作用下，转导ＨＳＶ－ＴＫ基因的膀胱癌细胞的存活率受到影响，ＡＣＶ可显著抑制癌细胞生长。结论：脂质体／ＴＫ重组 ＤＮＡ复合物可用于膀胱癌细胞的转基因治疗。 Objective: To investigate the transduction of liposome/HSV-TK recombinant DNA in bladder cancer cells and its active expression in cells. METHODS: The HSV-TK gene retrovirus recombinant (pLXSN-TK) was introduced into BIU87 bladder cancer cell line using liposome/DNA complexes. BIU87 cells (BIU87-TK) carrying the HSV-TK gene were screened by G418. After Southern hybridization and in situ hybridization, the effects of ACV on the survival of BIU87-TK cells were observed. Results: Southern blot hybridization confirmed that the HSV-TK gene was present in the chromosome of BIU87-TK cells. In situ hybridization was used to detect the expression of HSV-TK mRNA. The introduction of exogenous DNA and expression of HSV-TK and Neo genes did not alter the cell growth characteristics. However, under the influence of ACV, the survival rate of bladder cancer cells transduced with the HSV-TK gene is affected, and ACV can significantly inhibit the growth of cancer cells. Conclusion: The liposome/TK recombinant DNA complex can be used for the transgene treatment of bladder cancer cells.