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目的 探讨金属基质蛋白酶 (MMP)及其抑制物检测在判断膀胱癌恶性度及预后中的作用。 方法 采用Westernblot和RT PCR方法 ,检测人膀胱肿瘤组织中MMP 2、MMP 9、TIMP 2蛋白表达和MMP 2、TIMP 2基因表达 ,应用计算机图像分析测定表达电泳条带的吸光度A值。 结果 膀胱肿瘤组织MMP 2、MMP 9、TIMP 2蛋白与基因表达与正常膀胱组织比较 ,差别有显著性意义 (P <0 .0 1) ;膀胱肿瘤组织TIMP 2蛋白与基因的吸光度A平均值与正常膀胱组织比较 ,差别无显著性意义 (P >0 .0 5 )。表浅膀胱癌组织的MMP 2 /TIMP 2蛋白、基因吸光度A平均值比率与正常膀胱组织比较 ,差别有显著性意义 (P <0 .0 5 ) ;浸润性膀胱癌的MMP 2 /TIMP 2蛋白、基因吸光度A平均值比率与正常膀胱组织相比 ,差别有显著性意义 (P <0 .0 1)。G2 、G3 与G1的MMP 2 /TIMP 2平均吸光度A值比率差别有显著性意义 (P <0 .0 1)。 结论 MMP 2、MMP 9表达和MMP 2 /TIMP 2比率对判断膀胱肿瘤浸润及转移有一定意义。检测MMP 2 /TIMP 2比率失衡较单纯检测MMP 2、TIMP 2的蛋白与基因表达更有意义
Objective To investigate the role of matrix metalloproteinase (MMP) and its inhibitors in the diagnosis of malignancy and prognosis of bladder cancer. Methods The expressions of MMP 2, MMP 9, TIMP 2 and MMP 2, TIMP 2 in human bladder tumor were detected by Western blot and RT PCR. The absorbance value A of the expression band was determined by computer image analysis. Results The expressions of MMP 2, MMP 9 and TIMP 2 protein in bladder tumor tissues were significantly higher than those in normal bladder tissues (P <0.01). The average values of absorbance A and TIMP-2 in bladder tumor tissues were There was no significant difference between normal bladder tissues (P> 0.05). The ratio of MMP 2 / TIMP 2 protein and gene A absorbance in superficial bladder cancer tissues was significantly higher than that in normal bladder tissues (P <0.05), MMP 2 / TIMP 2 protein in invasive bladder cancer , The average ratio of gene absorbance A compared with normal bladder tissue, the difference was significant (P <0.01). There was a significant difference (P <0.01) in the average absorbance A ratio of MMP 2 / TIMP 2 between G2, G3 and G1. Conclusion The expressions of MMP 2 and MMP 9 and the ratio of MMP 2 / TIMP 2 have some significance in judging the infiltration and metastasis of bladder tumor. Detecting the imbalance of MMP2 / TIMP2 ratio is more meaningful than simply detecting the protein and gene expression of MMP2 and TIMP2