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目的观察三七皂甙 (PNS)及三七皂甙单体 (Rb 1、Rg 1)对大脑中动脉栓塞 (MCAO)后脑血流量(CBF)及脑组织超微结构的影响。方法60只Wistar大鼠随机分7组 :其中第7组为假手术对照组 (SO) ;第1组为MCAO对照组 ;第2 ,3 ,4组MCAO前30min及MCAO后即刻分别静脉给予Rg120μg·kg-1,Rb120μg·kg-1 ,Nim5μg·kg-1 ;第5 ,6组分别于MCAO前灌胃PNS200mg·kg-1 及400mg·kg-1 连续10d。结果与MCAO对照组比较Rb 1,Nim ,PNS400mg·kg-1 组 ,CBF明显增高 (P<0.01) ,Rg1 组CBF无明显增加。PNS能明显提高超氧化物歧化酶 (SOD)活力 ,降低一氧化氮 (NO)含量。超微结构显示 ,Rb1,Rg1,PNS和Nim能明显减轻脑缺血性神经元损伤 ,其中Rb1,Nim ,PNS400mg·kg-1组优于Rg1 和PNS200mg·kg-1组。结论Rb1 ,Nim和PNS400mg·kg-1能明显增加MCAO区CBF ,其机理可能与扩张血管有关 ;Rg1不能增加CBF。Rb 1,Rg 1,PNS有减轻脑缺血坏死作用。其机理可能与提高脑细胞内SOD活力、降低细胞内Ca2 +及NO含量有关。
Objective To observe the effects of panax notoginseng (PNS) and notoginsenoside (Rb 1 and Rg 1) on cerebral blood flow (CBF) and brain ultrastructure of middle cerebral artery occlusion (MCAO). Methods Sixty Wistar rats were randomly divided into 7 groups: the seventh group was sham-operated control group (SO); the first group was MCAO control group; the second, third, and fourth groups were administered intravenously 120g before MCAO and immediately after MCAO. · kg-1, Rb120μg·kg-1, Nim5μg·kg-1; Groups 5 and 6 were intragastrically administered with PNS 200mg·kg-1 and 400mg·kg-1 for 10 days before MCAO. Results Compared with MCAO control group, CBF was significantly increased in Rb 1, Nim and PNS 400 mg·kg-1 groups (P<0.01), while CBF in Rg1 group had no significant increase. PNS can significantly increase the activity of superoxide dismutase (SOD) and reduce the content of nitric oxide (NO). The ultrastructure showed that Rb1, Rg1, PNS and Nim could significantly reduce cerebral ischemic neuronal damage, among which Rb1, Nim, PNS400mg·kg-1 group was superior to Rg1 and PNS200mg·kg-1 group. Conclusions Rb1, Nim and PNS 400mg·kg-1 can significantly increase the CBF in MCAO region. The mechanism may be related to dilation of blood vessels. Rg1 can not increase CBF. Rb 1, Rg 1, and PNS can relieve cerebral ischemic necrosis. The mechanism may be related to the increase of SOD activity in brain cells and the decrease of intracellular Ca2 + and NO levels.