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目的:探讨第10号染色体同源缺失磷酸酶-张力蛋白基因(PTEN)、存活素(Sur-vivin)及环氧合酶-2(COX-2)在乳腺癌组织中的表达及其临床意义。方法:免疫组化Envision法检测112例乳腺癌组织中ER、PR、c-erbB-2、PTEN、Survivin及COX-2表达,检测20例乳腺增生症中PTEN、Sur-vivin及COX-2表达。结果:乳腺癌组织中PTEN、Survivin及COX-2的阳性表达率分别为48.2%(54/112)、59.8%(67/112)及61.6%(69/112),乳腺增生症中分别为90.0%(18/20),0(0/20)及15.0%(3/20)。PTEN表达与肿瘤组织学分级、临床分期、淋巴结转移以及ER、c-erbB-2、Survivin和COX-2表达均有明显相关性,P<0.01。Survivin表达与肿瘤大小、淋巴结转移以及ER、c-erbB-2和PTEN表达均有明显相关性,P<0.05。COX-2表达与肿瘤大小、淋巴结转移以及ERc、-erbB-2、PTEN表达均有明显相关性,P<0.05。PTEN表达与Sur-vivin、COX-2表达均呈显著负相关(P<0.01),而Survivin表达与COX-2表达呈显著正相关,P<0.01。结论:检测PTEN、Sur-vivin及COX-2基因蛋白的表达对于评估乳腺癌的生物学行为具有重要价值。PTEN表达下调与Survivin和COX-2表达上调可能在乳腺癌的发生和进展中发挥协同作用。
Objective: To investigate the expression of Survivin and Cyclooxygenase-2 (COX-2) on chromosome 10 in breast cancer and its clinical significance . Methods: The expressions of ER, PR, c-erbB-2, PTEN, Survivin and COX-2 in 112 cases of breast cancer were detected by immunohistochemical Envision method. The expressions of PTEN, Sur-vivin and COX- . Results: The positive rates of PTEN, Survivin and COX-2 in breast cancer tissues were 48.2% (54/112), 59.8% (67/112) and 61.6% (69/112), respectively, and those in breast hyperplasia were 90.0 % (18/20), 0 (0/20) and 15.0% (3/20). The expression of PTEN was correlated with tumor histological grade, clinical stage, lymph node metastasis and the expression of ER, c-erbB-2, Survivin and COX-2, P <0.01. Survivin expression was significantly correlated with tumor size, lymph node metastasis, ER, c-erbB-2 and PTEN expression (P <0.05). COX-2 expression was significantly correlated with tumor size, lymph node metastasis, ERc, -erbB-2 and PTEN expression (P <0.05). The expression of PTEN was negatively correlated with the expression of Survivin and COX-2 (P <0.01), while the expression of Survivin was positively correlated with the expression of COX-2 (P <0.01). Conclusion: Detecting the expression of PTEN, Sur-vivin and COX-2 gene proteins is of great value in assessing the biological behavior of breast cancer. Down-regulation of PTEN expression and up-regulation of Survivin and COX-2 may play synergistic roles in the occurrence and progression of breast cancer.