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电磁脉冲 (EMP)作为一种非电离辐射 ,生物效应广泛 ,从自由基生物学角度研究其对神经细胞的辐射损伤效应具有一定的实际意义。方法 采用自旋捕捉的方法检测自由基的变化 ,TBA法检测脂质过氧化产物丙二醛 (MDA) ,DTNB法检测谷胱甘肽过氧化物酶 (GSH px)的活性 ,MTT法检测细胞存活率 ,流式细胞术检测细胞内Ca2 + 浓度。结果 电磁脉冲作用大鼠大脑皮层细胞和海马细胞后 ,脂氧自由基水平升高。MDA水平升高 ,大脑皮层细胞对照组MDA水平为 (6 18± 0 2 9)nmol mg,EMP作用组为 (8 4 3± 0 0 1)nmol mg(P <0 0 1) ;海马细胞对照组MDA水平为 (4 38± 0 15 )nmol mg,EMP作用组为 (4 98± 0 39)nmol mg(P <0 0 5 )。GSH px活性降低 ,大脑皮层细胞对照组A值为 0 0 2 6± 0 0 0 1,EMP作用组为 0 0 2 2± 0 0 0 2 (P <0 0 5 ) ,海马细胞对照组A值为 0 0 2 1± 0 0 0 2 ,EMP作用组为 0 0 18± 0 0 0 1(P <0 0 5 )。电磁脉冲不能直接杀死细胞 ,其存活率约为 95 % ,当脉冲数达到 10次时 ,细胞内Ca2 + 浓度开始升高。结论 电磁脉冲提高了神经细胞脂氧自由基水平 ,脂质过氧化产物MDA水平升高 ,GSH px活性降低 ,预示氧化损伤效应的发生。另外 ,电磁脉冲不能直接杀死细胞 ,但可导致细胞内Ca2 + 浓度升高
Electromagnetic pulse (EMP), as a non-ionizing radiation, has a wide range of biological effects. It is of practical significance to study its radiation damage effect on nerve cells from the perspective of free radical biology. Methods The changes of free radicals were detected by spin-capture method. The malondialdehyde (MDA) was detected by TBA method, the activity of glutathione peroxidase (GSH px) by DTNB method, Survival rate, flow cytometry intracellular Ca2 + concentration. Results Electromagnetic pulse induced rat cerebral cortical cells and hippocampal cells, lipid-oxygen free radicals increased. The levels of MDA in the control group were (6 18 ± 0 2 9) nmol mg, and those in the EMP group were (843 ± 0 0 1) nmol mg (P 0 01) The level of MDA was (4 38 ± 0 15) nmol mg and the group of EMP was (4 98 ± 0 39) nmol mg (P 0 05). The activity of GSH px was decreased in control group (A 0 256 ± 0 0 0 1) and EMP-treated group (0 0 2 2 ± 0 0 0 2) (P 0 05) in hippocampus. Was 0 0 2 1 ± 0 0 0 2, and EMP-treated group was 0 0 18 ± 0 0 0 1 (P 0 05). Electromagnetic pulse can not directly kill cells, the survival rate of about 95%, when the pulse number reaches 10, the intracellular Ca2 concentration began to rise. Conclusion Electromagnetic pulse enhanced the level of lipoxygen free radicals in nerve cells, the MDA level of lipid peroxidation products increased and the activity of GSH px decreased, indicating the occurrence of oxidative damage. In addition, the electromagnetic pulse can not kill cells directly, but can lead to elevated intracellular Ca2 +