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目的探讨 IL-8启动子-251A/T 基因多态性与呼吸道合胞病毒(RSV)致毛细支气管炎(简称毛支)及毛支后婴幼儿喘息的相关性。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测320例 RSV 毛支患者及272例正常对照者 IL-8基因-251A/T 多态性,酶联免疫吸附试验(ELISA)法检测血清 IL-8、IgE 浓度。并对入组的毛支患儿随访3年,记录婴幼儿毛支后喘息再发的情况。结果 (1)RSV 毛支组和对照组 IL-8-251位 A 等位基因频率分别为45.6%、37.7%,两组间比较差异有统计学意义(P<0.05);(2)基因型 TT、AT、AA 的毛支患儿血清 IL-8浓度分别为(17±6)ng/L、(21±7)ng/L、(24±9)ng/L,三种基因型间比较差异有统计学意义(P<0.01);(3)RSV 毛支后喘息组和未再喘息组 IL-8-251位 A 等位基因频率分别为54.6%、35.8%,两组间比较差异有统计学意义(P<0.05)。结论 IL-8启动子-251A/T 基因多态性与 RSV 毛支易感性相关,且携带 IL-8-251A 等位基因的患儿在 RSV 毛支后更容易出现喘息。
Objective To investigate the association between the polymorphism of IL-8 promoter -251A / T gene and respiratory syncytial virus (RSV) induced bronchiolitis (bronchi branch) and infantile bronchial asthma after branch of hair. Methods The polymorphisms of IL-8 gene -251A / T in 320 patients with RSV bronchiolitis and 272 healthy controls were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Enzyme-linked immunosorbent assay (ELISA) method to detect serum IL-8, IgE concentration. The patients with bronchiolitis were followed up for 3 years and the recurrence of wheezing was recorded after the bronchial branches were inflated. Results (1) The allele frequencies of IL-8-251 A allele in RSV bronchiolitis group and control group were 45.6% and 37.7%, respectively, with statistical significance (P <0.05). (2) The genotype The levels of serum IL-8 in TT, AT and AA children were (17 ± 6) ng / L, (21 ± 7) ng / L and (24 ± 9) ng / L respectively (P <0.01). (3) The frequency of allele A of IL-8-251 in RSV group with or without resuscitation was 54.6% and 35.8%, respectively. There was significant difference between the two groups Statistical significance (P <0.05). Conclusion The polymorphism of IL-8 promoter -251A / T gene is associated with RSV susceptibility, and children with IL-8-251A allele are more prone to wheeze after RSV branch of hair.