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目的探讨食蟹猴脑缺血模型在人骨髓间充质干细胞(human bone marrow-derived mesenchymal stemcells,hBMSCs)移植后IL-10的表达及其对脑缺血损伤的保护作用。方法食蟹猴8只,在脑定位仪定位下,应用光化学法构建食蟹猴脑缺血模型,并将其随机分为高剂量治疗组、低剂量治疗组和模型组,分别在脑缺血部位附近注射高、低密度的hBMSCs和生理盐水。手术后,通过影像学、神经功能评分及组织病理学观察对hBMSC的治疗效果进行评价。并应用原位细胞凋亡检测的方法观察脑缺血周围神经细胞的凋亡情况。应用免疫组织化学、RT-PCR以及real-time PCR法检测检测脑损伤周围IL-10的表达水平。结果与模型组相比,hBMSC治疗组损伤部位周围细胞凋亡明显减少,免疫组织化学显示IL-10阳性细胞的数量及染色强度均较模型组明显升高,IL-10在mRNA水平表达也明显升高。结论hBMSCs对食蟹猴脑缺血模型具有修复作用,其治疗机制可能与促进炎症抑制因子IL-10的表达有关。
Objective To investigate the expression of interleukin-10 (IL-10) after cerebral cortex mesenchymal stem cells (hBMSCs) transplantation in cynomolgus monkey cerebral ischemia model and its protective effect on cerebral ischemia. Methods Eight cynomolgus monkeys were divided into high-dose treatment group, low-dose treatment group and model group under photolocalization to establish cerebral cAMP model. Near the site of injection of high and low density hBMSCs and saline. After surgery, the therapeutic effect of hBMSC was evaluated by imaging, neurological function score and histopathology. Apoptosis of peripheral nerve cells was observed by in situ apoptosis detection. Immunohistochemistry, RT-PCR and real-time PCR were used to detect the expression of IL-10 in brain tissue. Results Compared with the model group, the number of apoptotic cells in the hBMSC-treated group decreased significantly. The number and staining intensity of IL-10 positive cells in the hBMSC-treated group were significantly higher than those in the untreated group, and the expression of IL-10 at the mRNA level was also significant Rise. Conclusion hBMSCs can repair cerebral ischemia model in cynomolgus monkeys, and its therapeutic mechanism may be related to the promotion of the expression of IL-10.