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目的 研究胃癌组织中记忆T细胞及细胞粘附分子 (ICAM ) 1的表达 ,探讨其与TNM分期的关系。方法 正常胃粘膜组织 2 0例 ;胃癌 75例按TNM分期分为Ⅰ、Ⅱ、Ⅲ、Ⅳ组 ,标本经链霉素 生物素 过氧化物酶复合物 (SABC)免疫组织化学法染色后 ,对阳性结果进行分析。结果 正常胃粘膜组织中CD45RO (由记忆T细胞表达 )的表达率为 10 0 % ,阳性细胞数 ( 12 5 .7±14 .7) /HP ,ICAM 1表达率 95 .0 % ;胃癌组CD45RO表达率 44 .0 % ,阳性细胞数 ( 83 .7± 2 6.7) /HP ,ICAM 1表达率 3 7.3 % ,两组间差异有显著性 (P <0 .0 5 )。从胃癌Ⅰ至Ⅳ组 ,上述 3项值均呈下降趋势 ,且各组间差异均有显著性 (P <0 .0 5 )。结论 胃癌中记忆T细胞、ICAM 1表达率及阳性细胞数降低 ,提示T细胞有免疫耐受 ,机体对肿瘤的免疫杀伤作用下降 ,有利于判断机体的预后。
Objective To study the expression of memory T cell and cell adhesion molecule 1 (ICAM) 1 in gastric cancer and to investigate its relationship with TNM staging. Methods Twenty cases of normal gastric mucosa were divided into two groups. 75 cases of gastric cancer were divided into groups Ⅰ, Ⅱ, Ⅲ and Ⅳ according to TNM stage. The specimens were stained with streptavidin biotin peroxidase complex (SABC) Positive results were analyzed. Results The positive expression rate of CD45RO (memory T cells) in normal gastric mucosa was 100%, the positive cells were (125.7 ± 14.7) / HP, ICAM 1 was 95.0% The positive expression rate was 44.0%, the positive cell number was 83.7 ± 2 6.7 / HP and the ICAM 1 expression rate was 7.33%. There was a significant difference between the two groups (P <0.05). From gastric cancer Ⅰ to Ⅳ, the above three values showed a downward trend, and the differences between the groups were significant (P <0.05). Conclusion The expression of memory T cells, ICAM 1 and the number of positive cells in gastric cancer are decreased, suggesting that T cells have immune tolerance and the immunosuppressive effect on the tumor is declining, which is good for judging the prognosis of the patients.