目的:建立啤酒中腺嘌呤、鸟嘌呤、次黄嘌呤、黄嘌呤四种嘌呤组分的高效液相色谱测定方法。方法:啤酒样品脱气,用三氟乙酸和甲酸水解或者用高氯酸水解,调溶液pH值为4.0,上高效液相色谱仪测定分析。结果:啤酒样品中四种嘌呤的含量分别是腺嘌呤25.95 mg/L,鸟嘌呤21.60 mg/L,次黄嘌呤4.35 mg/L,黄嘌呤9.35 mg/L,回收率分别为95.8%、91.3%、90.6%和91.3%。结论:此方法简便可行,检测灵敏度、精密度均满足啤酒检测要求。 Objective: To establish a high performance liquid chromatographic method for the determination of four purine components in beer, including adenine, guanine, hypoxanthine and xanthine. Methods: Beer samples were degassed, hydrolyzed with trifluoroacetic acid and formic acid, or hydrolyzed with perchloric acid. The pH of the solution was adjusted to 4.0 and analyzed by high performance liquid chromatography. Results: The contents of four purines in beer samples were 25.95 mg / L of adenine, 21.60 mg / L of guanine, 4.35 mg / L of hypoxanthine and 9.35 mg / L of xanthine, respectively. The recoveries were 95.8% and 91.3% , 90.6% and 91.3% respectively. Conclusion: This method is simple and feasible, the detection sensitivity and precision meet the requirements of beer testing.