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目的:探讨三磷酸腺苷(ATP)对小鼠骨骼肌成纤维细胞的迁移作用及其可能机制。方法:细胞划痕实验检测1μM、10μM、100μMATP对NOR-10细胞愈合率的影响;细胞迁移小室实验检测空白对照组、100μM ATP组、30μM PPADS+100μM ATP组、100μM RB2+100μM ATP组细胞的迁移率。结果:细胞划痕实验及迁移实验表明高浓度ATP能够促进NOR-10细胞的迁移能力,100μM ATP促细胞迁移能力最强(P<0.05),并且其促迁移作用能被30μM PPADS,100μM RB2所抑制(P<0.05),但100μM RB2的抑制作用更强(P<0.05)。结论:高浓度ATP(>10μM)能够促进NOR-10细胞的迁移能力,并且其促迁移可能通过激活P2Y受体作用大于P2X受体。
Objective: To investigate the migration of mouse skeletal muscle fibroblasts induced by adenosine triphosphate (ATP) and its possible mechanism. Methods: The effect of 1μM, 10μM and 100μM ATP on the healing rate of NOR-10 cells was detected by cell scratch assay. The cell migration in the blank control group, 100μM ATP group, 30μM PPADS + 100μM ATP group, 100μM RB2 + 100μM ATP group Mobility. Results: Scratch assay and migration assay showed that high concentrations of ATP promoted the migration of NOR-10 cells. 100μM ATP had the strongest migration ability (P <0.05), and its migration ability was enhanced by 30μM PPADS and 100μM RB2 (P <0.05), but the inhibitory effect of 100μM RB2 was stronger (P <0.05). CONCLUSION: High concentration of ATP (> 10μM) can promote the migration of NOR-10 cells, and its pro-migration may be greater than that of P2X receptor by activating P2Y receptor.