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目的:研究17-甲氧基-7-羟基-苯并呋喃查尔酮(YLSC)对体内外血小板聚集和凝血功能的影响。方法:将50只SD大鼠随机分为5组:对照组(含0.5%DMSO的生理盐水),YLSC低、中、高剂量组(2.5,5,10 mg.kg-1),阳性药阿司匹林组(10 mg.kg-1)。尾静脉注射相应药物1周后,腹主动脉采血,分别加入二磷酸腺苷(ADP)、胶原和花生四烯酸(AA)诱导血小板聚集,测定大鼠体内外血小板聚集率;同时观察YLSC对大鼠凝血酶原时间(PT)、活化部分凝血激酶时间(APTT)及凝血酶时间(TT)的影响。结果:与对照组相比,YLSC能显著抑制ADP和胶原诱导的大鼠体内、外血小板最大聚集率(P<0.05或P<0.01),最大抑制率分别为38.4%,42.5%,对AA诱导的血小板聚集无明显抑制作用;YLSC能显著延长大鼠血浆TT,APTT(与对照组比较,P<0.05或P<0.01),对PT无显著影响。结论:YLSC具有对抗血小板聚集和抗凝血作用。
AIM: To investigate the effect of 17-methoxy-7-hydroxy-benzofuran chalcone (YLSC) on platelet aggregation and coagulation in vitro and in vivo. Methods: Fifty Sprague-Dawley rats were randomly divided into five groups: control group (0.5% DMSO), YLSC low, medium and high dose groups (2.5, 5 and 10 mg.kg-1), positive drug aspirin Group (10 mg.kg-1). One week after the tail vein injection, the abdominal aorta blood was drawn and ADP, collagen and arachidonic acid (AA) were respectively added to induce the platelet aggregation. The platelet aggregation rate in vivo and in vitro was measured. At the same time, YLSC Rat prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT). Results: Compared with the control group, YLSC significantly inhibited the maximum aggregation rate of platelet in vivo and in vitro induced by ADP and collagen (P <0.05 or P <0.01), and the maximum inhibitory rates were 38.4% and 42.5% (P <0.05 or P <0.01, respectively) compared with the control group, while no significant effect on PT. Conclusion: YLSC has anti-platelet aggregation and anticoagulant effects.