论文部分内容阅读
目的观察S100A2与胰腺癌继发耐放射细胞株的相关性。方法检测胰腺癌细胞株及其继发耐放射株中S100A2基因及蛋白质的表达水平,以及应用去甲基化试剂5-氮杂-2-脱氧胞苷处理耐放射细胞株后的改变,并检测相关放射敏感性的改变。结果胰腺癌细胞株SW1990耐放射株与亲本株相比S100A2的基因、蛋白质水平均明显较低(2~3倍),耐放射细胞株经5-氮杂-2-脱氧胞苷处理后S100A2的mRNA、蛋白质水平均较用药前明显升高(2~5倍),且处理后的细胞株耐放射性明显降低。结论S100A2在胰腺癌继发耐放射细胞株中的低表达可能是引起其继发耐放射特性的机制之一。
Objective To investigate the relationship between S100A2 and secondary radiation-resistant cell lines of pancreatic cancer. Methods The expression levels of S100A2 gene and protein in pancreatic cancer cell lines and their secondary radioimmunoassays were detected, and the changes of the expression of S100A2 gene and protein after treatment with 5-aza-2-deoxycytidine were measured. Related radiosensitivity changes. Results Compared with the parental strain, the gene and protein levels of S100A2 in pancreatic cancer cell line SW1990 were significantly lower (2 ~ 3 times) than that of the parental strain. The radiation-resistant cell line S100A2 treated with 5-aza-2-deoxycytidine mRNA and protein levels were significantly higher than before treatment (2 to 5 times), and the treated cell lines significantly reduced radioactive resistance. Conclusion The low expression of S100A2 in secondary pancreatic cancer resistant cell lines may be one of the mechanisms responsible for the secondary radiation resistance.