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目的 探讨人类白细胞抗原HLA DRB1, DQB1等位基因与大肠癌遗传关联性。方法 运用序列特异性引物聚合酶链反应 ,结合等位基因序列分析 ,检测无亲缘关系的湖北籍汉族健康人136名、大肠癌组 5 4例患者的HLA DRB1、 DQB1基因。结果 大肠癌患者与正常人比较 ,HLA DRB1 0 90 1等位基因分布频率明显增高 (0 2 315、0 1397,P =0 0 33) ;而 DRB1 0 80X等位基因频率明显降低 (0 0 0 93、0 0 80 9,P =0 0 0 71)。两组间HLA DRB1其余等位基因及HLA DQB1等位基因分布频率 ,差异均无显著意义。结论 HLA DRB1 0 90 1与人大肠癌呈正关联 ,而HLA DRB1 0 80X则与其负关联 ,上述两等位基因结构还得到基因序列分析印证
Objective To investigate the genetic association between human leukocyte antigen HLA DRB1 and DQB1 alleles and colorectal cancer. METHODS: HLA DRB1 and DQB1 genes were detected in 136 unrelated healthy Han nationality Han people and 54 human colorectal cancer patients using sequence-specific primer polymerase chain reaction and allele sequence analysis. Results The frequency of alleles of HLA DRB1 0 90 1 allele was significantly higher in patients with colorectal cancer than in normal controls (0 2 315, 0 1397, P =0 33). The frequency of DRB 1 0 80X allele was significantly lower (0 0 0 93, 0 0 80 9, P =0 0 0 71). There was no significant difference between the two groups in the distribution of HLA DRB1 alleles and HLA DQB1 allele frequency. Conclusion HLA DRB1 0 90 1 is positively correlated with colorectal cancer, while HLA DRB 1 0 80X is negatively correlated with it. The above two allele structures are also confirmed by gene sequence analysis.