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目的 观察、评价白细胞介素 12 (IL 12 )对结核分支杆菌感染小鼠细胞因子的影响和疗效。方法 将BALB/c小鼠 42只 ,制成小鼠结核分支杆菌感染模型 ,随机分为对照组 (PBS)和IL 12共两组 (每组 2 1只 )。给予PBS或IL 12治疗 ,检测血清γ干扰素 (IFN γ)、IL 4、IL 10水平 (ELISA法 )和器官菌落计数 ,观察治疗后生存率。结果 IL 12组小鼠无一死亡 ,肺、肝、脾菌落数分别为 (2 2 7±10 7)× 10 5cfu/ml,(4± 3)× 10 5cfu/ml和 (1 5± 0 5 )× 10 5cfu/ml,INF γ(10 90± 40 6 )pg/ml。PBS组 8只死亡 ,肺、肝、脾菌落数分别为 (6 5 9± 40 5 )× 10 5cfu/ml、(2 3± 19)× 10 5cfu/ml和 (10 8± 3 5 )× 10 5cfu/ml,IFN γ(5 48± 2 14)pg/ml,两组比较差异有显著性。而血清IL 4水平变化不大 ,两组比较差异无显著性。结论 IL 12诱导IFN γ产生 ,促进Th1细胞反应 ,改变了Th1/Th2平衡 ,对结核分支杆菌感染小鼠产生保护效应
Objective To observe and evaluate the effect of interleukin 12 (IL 12) on cytokines in mice infected with Mycobacterium tuberculosis. Methods Twenty-four BALB / c mice were made into a model of Mycobacterium tuberculosis infection in mice. They were randomly divided into control group (PBS) and IL 12 group (21 in each group). After treatment with PBS or IL 12, the levels of IFN-γ, IL-4 and IL-10 were measured and the colony counts were counted. The survival rate after treatment was observed. Results There was no death in IL 12 group, and the number of colonies in lung, liver and spleen was (22 7 ± 10 7) × 10 5 cfu / ml, (4 ± 3) × 10 5 cfu / ml and (15 ± 0 5 ) × 10 5 cfu / ml, INF γ (10 90 ± 40 6) pg / ml. The number of colonies in lung, liver and spleen was (6 5 9 ± 40 5) × 10 5 cfu / ml, (2 3 ± 19) × 10 5 cfu / ml and (10 8 ± 3 5) × 10 5cfu / ml, IFNγ (5 48 ± 2 14) pg / ml, the difference between the two groups was significant. The level of serum IL 4 changed little, no significant difference between the two groups. Conclusion IL-12 induces IFNγ production, promotes Th1 cell response, alters Th1 / Th2 balance, and has protective effect against Mycobacterium tuberculosis infection in mice