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目的通过构建纯化正常结肠上皮组织和结肠癌组织基因表达谱筛选结肠癌相关基因。方法应用激光捕获显微切割-线性扩增-全基因组基因芯片流程对结肠癌组织及其相应正常结肠上皮组织进行实质细胞纯化并构建基因表达谱,筛选差异表达基因。结果纯化正常结肠上皮细胞和结肠癌细胞分别有14 939和15 276条基因表达。肿瘤组织差异表达基因中4倍以上者208条,其中上调基因72条,下调基因136条。上调基因前20条和下调基因前20条中,10条基因的表达变化在结肠癌中的意义已被证实,另有13条基因的表达变化在其他肿瘤研究中与以前报道一致。结论细胞纯化技术结合基因芯片构建基因表达谱可准确、高效的筛选结肠癌差异基因。
Objective To screen colon cancer related genes by constructing gene expression profiles of normal colon epithelial tissues and colon cancer tissues. Methods The cells were purified by laser capture microdissection - linear amplification - genome-wide microarray and purified from the normal colon epithelial tissue. The gene expression profiles were screened and the differentially expressed genes were screened. Results There were 14 939 and 15 276 gene expressions in normal colon epithelial cells and colon cancer cells, respectively. Tumor tissue differentially expressed more than 4 times in 208 genes, including up-regulated genes 72, down-regulated genes 136. The significance of the expression change of 10 genes in the top 20 up-regulated genes and the top 20 down-regulated genes in colon cancer has been confirmed. The expression changes of the other 13 genes have been reported in other tumor researches. Conclusion Cell purification technology combined with gene chip to construct gene expression profile can accurately and efficiently screen differential genes of colon cancer.