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目的:建立垂盆草药材的DNA分子鉴定方法。方法:不同地区野外采集景天属垂盆草及其近缘药用植物共4种,分别提取总DNA,扩增cp DNA trn L-trn F序列,比对所有样品的该段核苷酸序列,设计特异性鉴别垂盆草及其近缘种的位点特异性引物。结果:垂盆草及其近缘易混淆品的trn L-trn F序列具有多个稳定的SNP位点;选取适宜的变异位点设计的2对特异性引物,在一定的退火温度,只有垂盆草扩增后产生预期长度的阳性扩增产物,而其他3种近缘种在同等条件下不能被有效扩增。结论:所设计的鉴别引物在适宜的反应条件下能特异性鉴别垂盆草及其近缘易混淆植物。
Objective: To establish DNA molecular identification method of sarmentosum. METHODS: Four species of Sedum albizziae and their medicinal plants were collected in different areas. Total DNA was extracted and the cp DNA trn L-trn F sequence was amplified. The nucleotide sequence of all the samples , A site-specific primer was designed to identify sarmentosum and its related species. Results: The trn L-trn F sequence of Saussurea indicus and its susceptible products had multiple stable SNP sites. Two pairs of specific primers designed by the appropriate mutation sites were only annealed at a certain annealing temperature The amplification of potted plants produced a positive amplification product of expected length, while the other three closely related species could not be efficiently amplified under the same conditions. CONCLUSION: The designed primers can identify sargassum and its closely related plants under suitable reaction conditions.