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报告了应用酶放大镧系元素发光法测定碱性磷酸酶的方法．应用５氟水杨酸磷酸酯作为酶底物，并对方法学中的多种因素进行了最佳比．用甲基硅油（Ｉ）改进了信／噪比的稳定性．方法的灵敏度为４Ｕ／Ｌ．精密度为１０％．精密度为１０％的浓度范围是２００～３００×１０２Ｕ／Ｌ．测量值的相对误差＜１０％．测定了血清中的碱性磷酸酶浓度，回收率为９３％～９５％．
A method for the determination of alkaline phosphatase using the enzyme lanthanide luminescence method is reported. Application of 5-fluorosalicylic acid phosphate as enzyme substrate, and the methodology of the various factors in the best ratio. Using methyl silicone oil (I) to improve the stability of the letter / noise ratio. The sensitivity of the method is 4U / L. Precision is 10%. The concentration range of 10% precision is 2 00 ~ 3 00 × 102U / L. The relative error of the measured value <10%. The concentration of alkaline phosphatase in the serum was measured and the recovery was 93% -95%.