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目的探讨苦参碱对人胃癌细胞株SGC-7901体外侵袭能力及乙酰肝素酶(HPSE)mRNA表达的影响作用。方法SGC-7901细胞体外培养,以0.125、0.25和0.5mg/m l不同浓度苦参碱进行预处理48h后,采用半定量RT-PCR检测各组培养细胞HPSE mRNA表达的变化,应用细胞黏附实验检测细胞黏附能力的改变,Transwell小室检测侵袭及趋化能力的变化。结果与对照组比较,各浓度苦参碱处理组SGC-7901细胞HPSE mRNA的表达和细胞黏附侵袭能力均受到明显的抑制(P<0.01),其抑制效应与药物的浓度呈正相关,组间比较,P<0.01。结论苦参碱可能通过下调SGC-7901细胞HPSE mRNA的表达而显著抑制细胞的体外侵袭能力,其抑制作用呈剂量依赖性。
Objective To investigate the effect of matrine on the invasiveness of gastric cancer cell line SGC-7901 in vitro and the expression of heparanase (HPSE) mRNA. Methods SGC-7901 cells were cultured in vitro and pretreated with different concentrations of matrine at 0.125, 0.25 and 0.5 mg / ml for 48 h. The changes of HPSE mRNA expression in each group were detected by semi-quantitative RT-PCR. Cell adhesion assay Changes in cell adhesion capacity, Transwell chamber detection of invasion and chemotactic capacity changes. Results Compared with the control group, the expression of HPSE mRNA and the ability of cell adhesion and invasion in SGC-7901 cells treated with matrine at all concentrations were significantly inhibited (P <0.01). The inhibitory effect was positively correlated with the drug concentration. , P <0.01. Conclusion Matrine may inhibit the invasion of cells in vitro by down-regulating the expression of HPSE mRNA in SGC-7901 cells in a dose-dependent manner.