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正常大豆Peking品种悬浮细胞具有典型的细胞结构,但经500拉德γ射线照射后,细胞结构受到辐射损伤,表现为质体、线粒体等细胞器相对减少,核膜扭曲、模糊。但经1个月继代培养后,细胞恢复正常的结构和功能。5000拉德γ射线能使大豆悬浮细胞产生致死损伤,导致细胞器解体和细胞死亡。本实验未能观察到辐射击穿细胞的直接证据,但出现膜渗透性改变、质壁分离及其内质网膨大等现象,间接证明了细胞自身防御体系被打破,有利于外源基因导入
The normal cell suspension cells of Peking cultivar have the typical cell structure. However, irradiated by γ-rays of 500 rad, the cell structure is damaged by radiation, showing the decrease of plastids, mitochondria and other organelles, and the nuclear membrane distorted and blurred. However, after one month of subculture, the cells returned to normal structure and function. 5000 rad γ-rays can cause lethal damage to soybean suspension cells, resulting in disorganization of organelles and cell death. This experiment failed to observe the direct evidence of radiation breakdown cells, but the membrane permeability changes, plasmolysis and endoplasmic reticulum swelling and other phenomena, an indirect proof of the cell’s own defense system is broken, is conducive to the introduction of foreign genes