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本研究针对黑麦可能提供白粉病新的抗源,对最近育成的一组小麦-黑麦附加系、代换系和易位系进行了分析.同时,对以上各系与一组带有抗性基因Pm1-9和Mlk的品种/品系进行了比较.四倍体和六倍体的Thatcher小麦,对小麦白粉病高度感染,而由四倍体Thatcher和Prolific黑麦衍生而来的小黑麦系,则高度抗病.黑麦染色体1R、4R和7R不决定供试的白粉病分离物的任何抗性.2D/2R代换系的白粉病抗性高度有效,而假设来源于2R染色体的Pm7基因,则主要表现为感病反应.对带有Pm7的Transec小麦进行细胞学分析(采用Giemsa C-显带技术进行染色体鉴定)的结果表明,“Transec”易位包括一条小麦染色体4B的完整短臂和该条染色体长臂大约一半的近端区以及由黑麦染色体5R的长臂远端区衍生来的黑麦片段.结构异染色质经不同的染色后,2D/2R代换系的染色体2R显示出特定的C-带型.黑麦染色体6R决定着对所有供试白粉病分离物的绝对抗性,同时还表明,这种抗性基因就位于6R染色体的长臂上.对于此种抗性,笔者提出了临时基因符号MlP6L.此外,笔者还提出了利用6BS/6RL小麦-黑麦易位系转易这种抗性基因的途径.
In this study, a new set of wheat-rye adventitious lines, substitution lines and translocation lines were analyzed in the light of the possibility that rye may provide a new source of powdery mildew resistance.At the same time, The genotypes / lines of the Pm1-9 and Mlk genes were compared Tetraploid and hexaploid Thatcher wheat were highly susceptible to wheat powdery mildew and the triticale derived from the tetraploid Thatcher and Prolific rye Lines were highly resistant to the disease.Rye chromosomes 1R, 4R and 7R did not determine any resistance of the tested powdery mildew isolates.The powdery mildew resistance of the 2D / 2R substitution lines was highly effective, whereas the rye chromosomes derived from chromosome 2R The Pm7 gene was predominantly susceptible.The results of cytological analysis of Transec wheat with Pm7 using the Giemsa C-banding technique showed that the “Transec ” translocation consisted of a wheat chromosome 4B Of the intact short arm and about half of the long arm of the chromosome and rye fragments derived from the long arm distal region of rye chromosome 5R.After different staining, the 2D / 2R substitution Department of chromosome 2R shows a specific C-band type rye chromosome 6R decision For all the absolute resistance of powdery mildew isolates, we also showed that this resistance gene is located on the long arm of chromosome 6 R. For this resistance, the author proposed a temporary gene symbol MlP6L.In addition, I also proposed Pathway for transfection of this resistance gene with 6BS / 6RL wheat - rye translocation line.