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目的:检测不同浓度和时间梯度的细菌脂多糖(LPS)诱导人鼻黏膜上皮细胞(HNE)环氧合酶-2(COX-2)表达和前列腺素E2(PGE2)释放状况,探讨其在鼻黏膜炎症发病机制中的作用。方法:应用Western印迹和荧光实时定量PCR技术检测LPS诱导下和COX-2特异性抑制剂阻断后,HNE中COX-2表达变化。以酶标免疫测定检测在此过程中PGE2的释放水平。结果:COX-2和PGE2在正常HNE中呈微弱的表达。LPS呈时间和浓度依赖性诱导HNECOX-2表达及PGE2释放;PGE2释放较COX-2晚;NS-398呈浓度依赖性减弱LPS对COX-2表达及PGE2释放的诱导。结论:LPS可有效诱导HNECOX-2表达和PGE2释放,PGE2由COX-2催化合成,COX-2和PGE2表达增加参与了LPS诱导HNE的体外炎症过程。
OBJECTIVE: To detect the expression of cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) in human nasal epithelial cells (HNE) induced by bacterial lipopolysaccharide (LPS) of different concentrations and time gradients, The role of mucosal inflammation in the pathogenesis. Methods: The expression of COX-2 in HNE was detected by Western blotting and real-time fluorescence quantitative PCR after LPS-induced and COX-2 specific inhibitor block. Enzyme immunoassay was used to detect the release of PGE2 during this process. Results: COX-2 and PGE2 were weakly expressed in normal HNE. LPS induced HNECOX-2 expression and PGE2 release in a time and concentration-dependent manner; PGE2 release was delayed compared with that of COX-2; and NS-398 attenuated LPS-induced COX-2 expression and PGE2 release in a dose-dependent manner. CONCLUSION: LPS can effectively induce the expression of HNECOX-2 and release of PGE2. PGE2 is catalyzed by COX-2. The expression of COX-2 and PGE2 is involved in the in vitro inflammatory process induced by LPS.