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探讨益气活血开窍复方脑脉康颗粒对缺血再灌注脑保护作用的机理。用线栓法制成大鼠脑缺血再灌注模型,采用全自动氨基酸分析仪、干湿重法、原子吸收分光光度计测量脑组织中兴奋性氨基酸（EAA）含量、含水量及钙离子（Ca2+）含量,HE法观察脑组织形态学改变。结果显示中药组谷氨酸（Glu）含量下降（P<0.01）,谷氨酸/γ-氨基丁酸（Glu/GABA）趋向于正常比值,脑组织含水量及Ca2+含量下降（P<0.05）。中药可减轻由于缺血再灌注所导致的脑细胞形态学的改变。证明益气活血开窍方脑脉康颗粒通过抑制EAA释放,Ca2+内流而发挥脑保护作用。
To explore the mechanism of Yiqi Huoxue Kaiqiao Fufang Naomaikang Granule on cerebral ischemia-reperfusion injury. The model of cerebral ischemia-reperfusion in rats was made by the thread plug method. The contents of excitatory amino acids (EAA), water content, and calcium ions in brain tissue were measured by automatic amino acid analyzer, wet-dry weight method and atomic absorption spectrophotometer (Ca2+). ) Content, HE method to observe the morphological changes of the brain. The results showed that the glutamate (Glu) content in the Chinese herbs group decreased (P<0.01), glutamic acid/gamma-aminobutyric acid (Glu/GABA) tended to normal ratio, and the brain tissue water content and Ca2+ content decreased (P<0.05). . Chinese herbs can reduce the changes of brain cell morphology caused by ischemia-reperfusion. It has been proved that Yi Qi Huo Xue Kai Fang Fang Nao Mai Kang Granule exerts brain protection by inhibiting EAA release and Ca2+ influx.