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探讨一组秸秆还田菌群ADS-3的功能稳定性及初步观察其接种于土壤后对还田秸秆的促腐效果。结果表明:在培养瓶中菌种ADS-3对小麦、水稻和玉米3大农作物秸秆都有稳定高效的分解能力,35℃条件下培养11d,能分解稻秆51.6%、小麦秸秆44.7%、玉米秸秆40.8%;在4、15和35℃条件下,尽管ADS-3对小麦秸秆的分解率差异很大,依次为6.8%、21.6%和44.7%;但是用变性梯度凝胶电泳(DGGE)检测其培养液,发现不同温度条件下ADS-3的微生物组成稳定。将菌种接种到模拟秸秆还田土壤中,初步试验结果表明ADS-3能显著提高秸秆的腐解速度(P<0.05)。试验结果为该菌群进一步开发秸秆还田促腐菌剂提供试验参数和依据。
To investigate the functional stability of ADS-3 in a group of straw returned to the soil and preliminary observation of its effect on the straw returning after inoculation into the soil. The results showed that Strain ADS-3 could stably and efficiently decompose three kinds of crop stalks, including wheat, rice and maize, at 35 ℃ for 11 days, which could decompose 51.6% of rice stalks, 44.7% of wheat stalks, The decomposition rate of wheat straw by ADS-3 varied greatly from 6.8%, 21.6% and 44.7% at 4, 15 and 35 ℃, respectively. However, the denaturing gradient gel electrophoresis (DGGE) Its culture medium, found under different temperature conditions ADS-3 microbial composition stability. The results showed that ADS-3 could significantly increase the decomposition rate of straw (P <0.05). The test results for the development of the flora to further promote the use of straw to promote fungal agents to provide experimental parameters and basis.