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目的观察皮肤鳞状细胞癌(鳞癌)和基底细胞癌(基癌)中细胞凋亡及其与c-fos、BNIP1表达的关系。方法应用原位末端标记和原位杂交技术检测48例皮肤鳞癌和41例基癌标本中细胞凋亡及C-fos、BNIP1 mRNA表达。结果鳞癌中凋亡指数(AI)、c-fos mRNA表达明显高于基癌(P＜0．01),但BNIP1 mRNA表达在二者间的差异无显著性(P＞0．05)。AI在高分化鳞癌中明显增多(P＜0．05),但C-fos表达以低分化鳞癌更为明显(P＜0．05)。鳞癌中AI与c-fos、BNIP1表达呈显著正相关(P＜0．05)。结论鱗癌中细胞凋亡增多与c-fos表达上调相关,BNIP1在鳞癌和基癌中可能起促凋亡作用。
Objective To observe the relationship between apoptosis and expression of c-fos and BNIP1 in squamous cell carcinoma (SCC) and basal cell carcinoma (SCC) of skin. Methods The expression of C-fos and BNIP1 mRNA in 48 cases of squamous cell carcinoma and 41 cases of basic carcinoma were detected by in situ end-labeling and in situ hybridization. Results The apoptosis index (AI) and the expression of c-fos mRNA in squamous cell carcinoma were significantly higher than those in the basal carcinoma (P <0.01). However, there was no significant difference in BNIP1 mRNA expression between the two groups (P> 0.05). AI was significantly higher in well-differentiated squamous cell carcinoma (P <0.05), but the expression of C-fos was more marked in poorly differentiated squamous cell carcinoma (P <0.05). There was a significant positive correlation between AI and c-fos and BNIP1 in squamous cell carcinoma (P <0.05). Conclusion The increased apoptosis in squamous cell carcinoma correlates with the up-regulation of c-fos expression. BNIP1 may play a role in promoting apoptosis in squamous cell carcinomas and carcinomas.