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目的研究irisin对C2C12小鼠骨骼肌细胞脂肪酸氧化的影响及机制。方法将培养好的C2C12细胞分为6组:对照组,irisin(20 ng/ml)组,irisin(200 ng/ml)组,irisin(2 000 ng/ml)组,irisin(2 000 ng/ml)+compound C(irisn孵育前30 min,20μmol/L)组,AICAR(AMPK激动剂,10μmol/L)组。使用3H标记的油酸孵育C2C12细胞,通过检测代谢产物放射性活度反映脂肪酸氧化水平;Western blot检测C2C12细胞与骨骼肌中AMPK和ACC-β的磷酸化水平。结果 1与对照组相比,irisin组C2C12细胞脂肪酸氧化水平升高(P<0.05)。2与对照组相比,irisin组AMPK和ACC-β的磷酸化水平增高(P<0.05或P<0.01)。3与Irisin组相比,irisin+compound-C组C2C12细胞脂肪酸氧化水平以及AMPK和ACC-β的磷酸化水平明显降低(P<0.05或P<0.01)。结论 Irisin通过激活AMPK促进小鼠骨骼肌细胞脂肪酸氧化。
Objective To investigate the effect and mechanism of irisin on fatty acid oxidation in C2C12 mouse skeletal muscle cells. Methods The cultured C2C12 cells were divided into 6 groups: control group, irisin group (20 ng / ml), irisin group (200 ng / ml), irisin group ) + compound C (30μmol / L 30min before irisn incubation), AICAR (10μmol / L AMPK agonist). The 3H-labeled oleic acid was used to incubate C2C12 cells, and the level of fatty acid oxidation was measured by measuring the radioactivity of metabolites. The phosphorylation of AMPK and ACC-β in C2C12 cells and skeletal muscle was detected by Western blot. Results 1 Compared with the control group, the level of fatty acid oxidation in C2C12 cells of irisin group increased (P <0.05). Compared with the control group, the phosphorylation of AMPK and ACC-β in irisin group increased (P <0.05 or P <0.01). Compared with Irisin group, the levels of fatty acid oxidation and the phosphorylation of AMPK and ACC-β in irisin + compound-C group were significantly decreased (P <0.05 or P <0.01). Conclusion Irisin can promote fatty acid oxidation of skeletal muscle cells in mice by activating AMPK.