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为了探讨酿酒葡萄在埋土越冬期的蔗糖代谢机制,以西北地区主栽品种‘赤霞珠’为试材,检测了冬季下架埋土后根系和枝条可溶性糖含量和蔗糖代谢相关酶活性的变化。结果表明:在12月和1月的冰冻温度下‘赤霞珠’根系、枝条中的可溶性糖均显著积累,且还原糖总量高于蔗糖总量,此阶段还原糖在‘赤霞珠’抗冻反应中起主要的渗透调节作用;在低温非冰冻温度的3月,根系和枝条的蔗糖总量高于还原糖总量,此阶段蔗糖是‘赤霞珠’抗寒响应的主要可溶性糖。相关性分析表明,蔗糖、果糖含量与SPS、SS活性呈极显著正相关,葡萄糖含量与SS、AI活性呈极显著正相关。与根系比较,枝条中蔗糖代谢相关的生理指标与越冬低温的关系更为密切,除NI活性外的其他生理指标均与温度呈显著负相关,其中可溶性糖含量和SPS、AI活性与温度呈极显著负相关。
In order to explore the mechanism of sucrose metabolism of wine grape during the overwintering period, the main cultivars ’Cabernet Sauvignon’ in northwestern China were used as test materials to measure the soluble sugar content and enzyme activities related to sucrose metabolism Variety. The results showed that the soluble sugar content in roots and shoots of ’Cabernet Sauvignon’ significantly increased at the freezing temperatures of December and January, and the total amount of reducing sugars was higher than the total amount of sucrose. During this period, In March, the total amount of sucrose in roots and shoots was higher than the total amount of reducing sugars. In this stage, sucrose was the main soluble sugar of ’Cabernet Sauvignon’ . Correlation analysis showed that the content of sucrose and fructose was significantly and positively correlated with the activities of SPS and SS, and there was a significant positive correlation between glucose and SS and AI activity. Compared with the root system, the physiological indices related to sucrose metabolism in shoots were more closely related to the overwintering low temperature. Other physiological indices except NI activity showed a significant negative correlation with temperature, of which soluble sugar content, SPS, AI activity and temperature were extremely Significant negative correlation.