论文部分内容阅读
本实验以泡桐丛枝植原体为材料,运用差速离心和脉冲场电泳(Pulsed Field Gel Electrophoresis,PFGE),获得了无寄主DNA污染、完整的泡桐丛枝植原体染色体,并用Southern blot杂交进行了验证,建立了植原体全基因组的研究方法。同时对泡桐丛枝植原体染色体进行内切酶I-CeuI不完全酶切,测定了染色体全长,并定位了2个非连锁的rRNA操纵子在染色体上的位置,完成了泡桐丛枝植原体部分物理图谱的构建。实验结果表明,泡桐丛枝植原体染色体全长约为1 100kb,2个rRNA操纵子在染色体上相距500～600kb。
In this experiment, Pamputophyta dicotyledon phytoplasma was used as material. Chromosomes without host DNA and intact P. paulownia were obtained by differential centrifugation and pulsed field gel electrophoresis (PFGE), and Southern blotting was performed Validation, the establishment of phytoplasma genome-wide research methods. In the meantime, the full-length chromosome was detected by I-CeuI restriction endonuclease digestion of the paulownia witches broom phytoplasma chromosome, and the positions of two non-linked rRNA operons on the chromosome were located. Part of the physical map construction. The experimental results show that the total length of the chromosome of Pamput flora is about 1 100 kb, and the two rRNA operons are 500-600 kb apart on the chromosome.