体外分离、培养rBMSC,经细胞表面标记物检测和体外诱导分化鉴定后,分别采用MTT法检测PRP对细胞增殖活性的影响;细胞化学法检测PRP对成骨诱导后rBMSC碱性磷酸酶(alkaline phosphate,ALP)活性的影响;RealTime-PCR检测PRP对细胞成骨分化指标Col-3、OPN mRNA表达水平的影响;扫描电镜观察PRP对rBMSC在Bio-Oss上粘附的影响。结果:rBMSC传代后细胞生长状态相对稳定,呈梭形;细 The rBMSCs were isolated and cultured in vitro. The cell surface markers and in vitro differentiation were detected by MTT assay. The effect of PRP on the cell proliferation was detected by cytochemistry. The effect of PRP on the activity of rBMSC alkaline phosphatase , ALP). The effect of PRP on osteoblast differentiation index Col-3 and OPN mRNA expression was detected by RealTime-PCR. The effect of PRP on the adhesion of rBMSC on Bio-Oss was observed by scanning electron microscopy. Results: The growth of rBMSCs after passage was relatively stable and fusiform;