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采用胎盼蓝染色法，３Ｈ－ＴｄＲ掺入试验和流式细胞计数分析技术研究了ｃ－ｍｙｃｍＲ－ＮＡ帽区反义寡脱氧核苷酸对人乳腺癌ＭＣＦ－７细胞系增殖及其ｃ－ｍｙｃ蛋白表达的影响，结果表明：ｃ－ｍｙｃ反义寡脱氧核苷酸能抑制人乳腺癌ＭＣＦ－７细胞系生长和增殖，这种抑制作用有明显的剂量依赖性，同时也能抑制ＭＣＦ－７细胞ｃ－ｍｙｃ蛋白的表达。此外，本文对ｃ－ｍｙｃ基因调控细胞增殖及反义核苷酸的可能机制进行了探讨。
Fetal blue staining, 3H-TdR incorporation assay and flow cytometric analysis were used to investigate the proliferation of c-mycmR-NA cap antisense oligodeoxynucleotide on human breast cancer MCF-7 cell line and its c- The effect of myc protein expression showed that c-myc antisense oligodeoxynucleotide can inhibit the growth and proliferation of human breast cancer MCF-7 cell line. This inhibition has a significant dose-dependent, but also can inhibit MCF- 7 Cell c-myc protein expression. In addition, the possible mechanisms of c-myc gene regulation of cell proliferation and antisense nucleotides are discussed.