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HKT类基因是与植物耐盐性密切相关的一类基因。在作物中HKT蛋白可通过排出Na+来维持植物体内的Na~+/K~+平衡,从而影响植物耐盐性。通过在烟草中过表达玉米ZmHKT1;5基因,验证该基因具有提高植物耐盐性的作用。结果表明,过表达ZmHKT1;5基因的T0代材料即显示出叶片耐盐能力的明显提高;T2代转基因株系种子在含盐培养基上的发芽能力明显强于野生型材料,T2代转基因株系幼苗阶段的耐盐能力也得到了明显的提高。通过比较在盐胁迫后2月龄的转基因材料和野生型材料的生理指标,发现野生型材料中MDA和H_2O_2的含量相较转基因材料发生了更为明显的上升,说明转基因材料中过表达ZmHKT1;5基因有效降低了盐胁迫引起的过氧化物积累。综合转基因验证的结果,证明ZmHKT1;5基因具有提高植物耐盐性的作用。
HKT genes are closely related to plant salt tolerance of a class of genes. In crops, HKT protein can maintain plant Na + / K + balance by excreting Na +, thus affecting plant salt tolerance. Through overexpression of ZmHKT1; 5 gene in tobacco, it was verified that this gene has the effect of improving plant salt tolerance. The results showed that the T0 generation of overexpression ZmHKT1; 5 gene showed a significant increase in salt tolerance; T2 generation of transgenic lines of seed germination ability was significantly stronger in salt medium than wild-type material, T2 generation transgenic lines Department of seedling stage of salt tolerance has also been significantly improved. The results showed that the content of MDA and H_2O_2 in wild-type material was significantly higher than that in transgenic material by comparing the physiological indexes of transgenic materials and wild-type materials at 2 months after salt stress, indicating that ZmHKT1 was overexpressed in the transgenic material; 5 gene effectively reduces the peroxide accumulation caused by salt stress. Based on the results of transgenic validation, it was proved that ZmHKT1; 5 gene has the function of improving the salt tolerance of plants.