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目的研究自身免疫性甲状腺病（ＡＩＴＤ患者ＴＳＨ受体抗体（ＴＲＡｂ的异质性。方法选择异质性ＴＲＡｂ患者６例，用ＥＢ病毒转染其外周血Ｂ淋巴细胞并筛选能产生单克隆ＴＲＡｂ的阳性细胞孔。结果６例患者的ＴＳＨ结合抑制免疫球蛋白（ＴＢＩＩ）测定值为５１％～８９％，患者１、２、４、５、６存在甲状腺兴奋性抗体（ＴＳＡ），而患者１、２、３可测得甲状腺阻断性抗体（ＴＳＢＡｂ）。以ＥＢ病毒转染外周血Ｂ淋巴细胞后，患者３有５个细胞孔能产生ＴＢＩＩ，患者６有４个细胞孔能产生ＴＳＡｂ。ＴＲＡｂ的测定值存在量效关系。结论ＡＩＴＤ患者可产生兴奋性或抑制性ＴＳＨ受体抗体，ＴＲＡｂ存在异质性。
Objective To study the heterogeneity of TSA receptor antibody (TRAb) in patients with autoimmune thyroid disease (AITD) .Methods Six patients with heterogeneous TRAb were selected and their peripheral blood B lymphocytes were transfected with Epstein-Barr virus Positive cells.Results TSH binding inhibitory immunoglobulin (TBII) in 6 patients was 51% ~ 89%, and thyroid excitability antibody (TSA) was detected in patients 1, 2, 4, 2 and 3 can detect thyroid blocking antibodies (TSBAb). After transfection of peripheral blood B lymphocytes with Epstein-Barr virus, Patient 3 has 5 cell wells producing TBII and 6 of 6 cells producing TSAb. TRAb Dose-effect relationship.Conclusion AITD patients can produce excitatory or inhibitory TSH receptor antibodies, TRAb heterogeneity.