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探讨小续命汤提取物(Xiao-Xu-Ming decoction extract,XXM)对脂多糖(lipopolysaccaride,LPS)诱导的体内外神经炎症的影响.体外实验应用200 ng/mLLPS处理BV2小胶质细胞24小时以诱导炎症反应.体内实验应用5 mg/kg LPS诱导小鼠炎症反应.Griess试剂测定NO含量;ELISA法测定IL-1β、IL-6、TNF-α和MCP-1的含量;WB检测lba-1、TLR4和MyD88蛋白的表达;RT-PCR测定TLR4和MyD88的mRNA水平.结果发现,在体外细胞上,XXM显著降低LPS诱导的BV2细胞上清液中NO、IL-1β、IL-6和TNF-α的水平增高,抑制LPS诱导的BV2细胞中炎症蛋白TLR4和MyD88的表达.在小鼠脑皮层组织中,XXM显著抑制LPS诱导的小胶质细胞活化,降低LPS诱导的炎症因子和趋化因子IL-1β、IL-6、TNF-α和MCP-1的水平增高,抑制LPS诱导的TLR4和MyD88蛋白的表达.结果表明,XXM可下调TLR4/MYD88信号通路来减轻LPS诱导的神经炎症反应.“,”In the present study,we aimed to investigate the effects of Xiao-Xu-Ming decoction extract (XXM) on lipopolysaccaride (LPS)-induced neuroinflammation in vitro and in vivo.In vitro,the microglia BV2 cells were treated with 200 ng/mL LPS for 24 h to induce inflammatory responses.In vivo,mice were treated with 5 mg/kg LPS to induce inflammatory responses.The NO level was determined by Griess Reagents.The levels of IL-1 β,IL-6,TNF-α and MCP-1 were determined by ELISA.The expressions of Iba-1,TLR4 and MyD88 at the protein levels were determined by Western blotting analysis.The mRNA levels of TLR4 and MyD88 were determined by real-time PCR.In vitro,XXM significantly reduced the levels of various pro-inflammatory factors,including NO,IL-1β,IL-6 and TNF-α,induced by LPS in the supernatant of BV2 cells and suppressed expressions of inflammatory proteins TLR4 and MyD88 induced by LPS in BV2 cells.In vivo,XXM significantly inhibited microglia activation,attenuated LPS-induced inflammatory factors and chemokine production,such as IL-1β,IL-6,TNF-α and MCP-1,and inhibited the expressions of inflammatory proteins including TLR4 and MyD88,in the cortex of LPS-induced mice.Our findings suggested that XXM could attenuate LPS-induced neuroinflammation via down-regulating TLR4/MyD88 signaling pathway.