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按堀江法分离人嗜酸细胞,混悬于含10％胎牛血清、庆大霉素(50μg/ml)的RPMI1640培养液中(1×10~6/ml)。将柴朴汤提取剂5g溶解于5ml蒸馏水中,离心后取上清,
Human eosinophils were isolated by Minjiang method and suspended in RPMI1640 medium (1×10-6/ml) containing 10% fetal bovine serum and gentamycin (50 μg/ml). Dissolve 5 g of Chai Pu Tang extract in 5 ml of distilled water and take the supernatant after centrifugation.