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目的了解黑龙江省内部分人免疫缺陷病毒1型(HIV-I)的亚型及基因序列特征。方法用巢式聚合酶链反应(nested-PCR),对黑龙江省内17份HIV-1感染者外周血单个核细胞(PBMC)中前病毒脱氧核糖核酸的膜蛋白(e(?))基因进行扩增,并对C2-V3的核苷酸序列进行测定和分析。结果系统树分析显示,17份样本中病毒与HIV泰国B(B′)亚型聚在一起,基因离散率为(6.194±1.01)%,与欧美B亚型基因离散率为(12.94±2.19)%,与其他亚型的离散率大于20%。对于其V3环四肽序列的分析表明,具有GPGQ的8例,占47.06%;具有GPGR的7例,占41.18%;1例为GQGR;1例为GPGH。通过序列分析预测,大部分利用CCR5辅助受体。结论所检测的黑龙江省17例HIV- 1均为B′亚型,提示黑龙江省的HIV-1流行株可能以B′亚型为主,其V3环顶端四肽序列特征以GPGQ和GPGR为主。
Objective To understand the subtype and gene sequence of HIV-I in Heilongjiang Province. Methods Using nested-PCR, the membrane protein (e (?)) Gene of provirus DNA in peripheral blood mononuclear cells (PBMCs) from 17 HIV-1 infected persons in Heilongjiang Province Amplified, and the C2-V3 nucleotide sequence was determined and analyzed. Results The phylogenetic tree analysis showed that the virus was clustered with HIV B (B ’) subtype in 17 samples, the gene dispersal rate was (6.194 ± 1.01)%, and the gene isolation rate with European and American subtype B was (12.94 ± 2.19) %, With other subtype dispersion rate greater than 20%. Analysis of its V3 loop tetrapeptide sequence revealed 8 cases with GPGQ, accounting for 47.06%, 7 cases with GPGR, accounting for 41.18%, 1 case with GQGR, and 1 case with GPGH. Prediction by sequence analysis, the majority of CCR5-assisted receptors. CONCLUSION: The 17 HIV-1 isolates tested in Heilongjiang Province are all B ’subtypes, suggesting that the HIV-1 epidemic strain in Heilongjiang Province may be mainly B’ subtype. The top tetrapeptide sequence of V3 loop is characterized by GPGQ and GPGR .