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目的:非整倍体是肿瘤细胞的典型特征,是人类肿瘤染色体不稳定性的普遍形式。中心体复制异常是肿瘤细胞中染色体不稳定性的主要原因。研究观察ECRG 2对中心体复制及染色体不稳定性的影响。方法:利用基因敲除和免疫荧光染色技术观察了ECRG 2基因对中心体复制和染色体不稳定性的影响。结果:s iRNA技术敲除ECRG 2基因导致中心体过度复制、多极纺锤体出现,最终导致染色体不稳定性和非整倍体细胞出现。结论:ECRG 2在中心体复制、纺锤体形成中起重要作用,对确保染色体稳定性必不可少;ECRG 2基因的缺失可能是肿瘤细胞染色体不稳定性和非整倍体细胞产生的重要原因。
Aims: Aneuploidy is a typical feature of tumor cells and a common form of chromosomal instability in human tumors. Centrosome replication anomalies are the major cause of chromosomal instability in tumor cells. To study the effects of ECRG 2 on centrosome replication and chromosome instability. Methods: The effects of ECRG 2 gene on centrosome replication and chromosomal instability were observed by gene knockout and immunofluorescence staining. RESULTS: Knockdown of the ECRG2 gene by siRNA resulted in over-replication of centrosomes and the appearance of multipolar spindles, eventually leading to chromosomal instability and aneuploidy. CONCLUSION: ECRG 2 plays an important role in centrosome replication and spindle formation, which is essential for ensuring chromosome stability. The loss of ECRG 2 gene may be an important reason for the chromosomal instability and aneuploidy of tumor cells.