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目的 :观察幼鼠致后海马的组织病理学改变。方法 :采用氯化锂 匹罗卡品腹腔注射制成幼鼠癫持续状态模型 ,应用常规病理及电镜观察海马结构的形态学改变 ,同时应用Timm组织化学染色方法进行苔藓纤维发芽的研究。结果 :海马区神经元可见变性、坏死的改变 ,以CA1区、CA3区为重。Timm染色见齿状回内分子层和CA3区下锥体层苔藓纤维发芽增加。结论 :①氯化锂 匹罗卡品诱导的幼鼠癫持续状态可造成海马区神经元损伤 ;②幼鼠癫持续状态后海马CA1、CA3区神经元损伤较重 ;③幼鼠癫持续状态后可致苔藓纤维发芽增加。
OBJECTIVE: To observe the histopathological changes of hippocampus in young rats after induced. Methods: The morphological changes of hippocampal formation were observed by routine pathology and electron microscopy. The morphological changes of hippocampal formation were observed by using intraperitoneal injection of pilocarpine hydrochloride. Meanwhile, Timm histochemical staining was used to study the mossy fiber sprouting. Results: Neurons in the hippocampus showed degeneration and necrosis changes, with CA1 and CA3 areas as the most important. Timm staining shows that germination of the mossy fiber in the dentate gyrus innerlayer and the pyramidal layer in the CA3 region is increased. CONCLUSION: ① The persistence of epilepticus induced by Pilocarpine in rats can cause neuronal damage in the hippocampus. ② The neurons in hippocampal CA1 and CA3 areas are severely damaged after the epilepticus is maintained. Sustained state can cause moss fiber sprouting increased.