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Diode-pumped acousto-optic Q-switched pulse laser at 1.5–1.6 \mu m is obtained in an Er3 :Yb3 :LuAl3(BO3)4 crystal. Single-pulse laser operation with slope efficiency of 14% and threshold of approximately 100 mW is realized at an average absorbed pump power of 314 mW and repetition frequency of 20 kHz. Output pulse energy is 67 \mu J. The effects of pulse repetition frequency, absorbed pump power, and duty cycle on the wavelength and pulse profile of the Q-switched Er3 :Yb3 :LuAl3(BO3)4 laser are also investigated.