在微生物学、免疫学及细胞工程等学科中经常遇到在同一标本中同时测定不同种类的抗原或其他生物因子的情况,因此产生了多重染色方法,如免疫荧光双染色法等。随着免疫酶方法应用的日趋广泛,免疫酶多重染色方法的建立是必要的。在做免疫酶多重染色实验中,即可以用不同种类酶标抗体在同一标本中 In the disciplines of microbiology, immunology, and cell engineering, it is common to find cases where different types of antigens or other biological factors are simultaneously detected in the same specimen, thus resulting in multiple staining methods, such as immunofluorescence double staining. With the increasing use of immunoenzymatic methods, the establishment of multiplexed immunostaining methods is necessary. In the immunostaining multiple staining experiment, different types of enzyme-labeled antibodies can be used in the same sample.