近十年来 ,各种功能性低聚糖的研究开发引起国内外学术界的普遍关注。从食品工业角度看 ,作为一种大量使用的功能性基料 ,采用酶法水解或酶法转移来生产各种低聚糖非常实用。文章主要利用微生物酶法合成低聚糖所存在的相关问题及策略 ,涉及酶法合成低聚糖的酶源、底物特异性、 In recent ten years, the research and development of various functional oligosaccharides have drawn the widespread attention of academics at home and abroad. From the food industry point of view, it is very practical to produce various oligosaccharides by enzymatic hydrolysis or enzymatic transfer as a large-scale functional base material. In this paper, the main problems related to the synthesis of oligosaccharides by using microbial enzymatic methods and strategies are discussed, including enzyme source, substrate specificity,