[Objectives] To establish a simple and rapid,specific and reproducible thin layer chromatography( TLC) method,and accordingly establish a thin layer identification method for quality control of Glycyrrhiza uralensis. [Methods] According to the physical and chemical properties,components of Glycyrrhiza uralensis were identified by thin layer chromatography. According to Veterinary Pharmacopoeia of the People’s Republic of China 2010( Volume Ⅱ) and related literature report,the thin layer chromatography identification method was studied and improved for Glycyrrhiza uralensis.[Results] When the preparation method for test sample solution was 6g Glycyrrhiza uralensis added with 30 mL saturated n-butanol,ultrasonic processing 20 min,filtered,filtrate dried,added 3 mL methanol and obtained Glycyrrhiza uralensis test sample solution. Then,chloroform-methanol-formic acid-ethyl acetate( 10∶ 3∶ 2∶ 3) as developing solvent,clear spot with high degree of separation and reproducibility could be obtained,and can be used for thin layer chromatography identification of ammonium glycyrrhetate in Glycyrrhiza uralensis.[Conclusions] The modified method omitted the step of ether treatment of samples,and directly adopted saturated n-butanol to extract components. It does not influence the detection of chromatographic spots,and solves the issue of sample gelatinizing sticking and filtration,simplifies the test process,and reduces the harm to human body. [Objectives] To establish a simple and rapid, specific and reproducible thin layer chromatography (TLC) method, and accordingly establish a thin layer identification method for quality control of Glycyrrhiza uralensis. [Methods] According to the physical and chemical properties, components of Glycyrrhiza uralensis were identified by thin layer chromatography. According to Veterinary Pharmacopoeia of the People’s Republic of China 2010 (Volume II) and related literature report, the thin layer chromatography identification method was studied and improved for Glycyrrhiza uralensis. [Results] When the preparation method for Test sample solution was 6g Glycyrrhiza uralensis added with 30 mL saturated n-butanol, ultrasonic processing 20 min, filtered, filtrate dried, added 3 mL methanol and obtained Glycyrrhiza uralensis test sample solution. Then, chloroform-methanol-formic acid- 10:3 2:3) as developing solvent, clear spot with high degree of separation and reproducibility coul [Conclusions] The modified method omitted the step of ether treatment of samples, and can be used for thin layer chromatography identification of ammonium glycyrrhetate in Glycyrrhiza uralensis. [Conclusions] The modified method omitted the step of ether treatment of samples, and directly passed saturated n-butanol to extract components. the detection of chromatographic spots, and solves the issue of sample gelatinizing sticking and filtration, simplifying the test process, and reduces the harm to human body.