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目的:建立毛细管气相色谱法同时测定透骨灵橡胶膏中樟脑、冰片和薄荷脑含量的方法。方法:以萘为内标物,采用内标物预先加入法,使用挥发油测定器制备供试液。以HP-5毛细管色谱柱为固定相,氮气为载气,FID检测器,程序升温法。结果:在该色谱条件下,樟脑、冰片和薄荷脑及内标物均得到良好的分离效果。樟脑、冰片和薄荷脑的加样回收率(n=6)分别为98.2%(RSD为0.42%)、102.4%(RSD为1.08%)和101.0%(RSD为2.34%)。结论:本方法灵敏、准确、重复性好,可以用于该制剂的质量控制。
OBJECTIVE: To establish a method for the simultaneous determination of camphor, borneol and menthol in Toujingling rubber paste by capillary gas chromatography. Methods: With naphthalene as the internal standard, the internal standard was added beforehand, and the volatile oil was used to prepare the test solution. HP-5 capillary column as stationary phase, nitrogen as carrier gas, FID detector, temperature-programmed method. Results: Under the chromatographic conditions, camphor, borneol, menthol and internal standard were well separated. The recoveries (n = 6) for camphor, borneol and menthol were 98.2% (RSD 0.42%), 102.4% (RSD 1.08%) and 101.0% (RSD 2.34%), respectively. Conclusion: The method is sensitive, accurate and reproducible and can be used for the quality control of the preparation.