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SL/SLd 是造血微环境有缺陷的小鼠。因此,不论在体内或体外,都可见有成熟造血细胞生成减少。本文目的是研究多照射与不照射的正常小鼠骨髓基质纠正,这种缺陷并在体外支持SL/SLd 骨髓细胞长期造血的能力。将接种有16×10~6个正常WCB_6F_1小鼠(+/+)或WCB_6F_1-SL/SLd 小鼠骨髓细胞的培养物进行长期培养。在单独进行的15次实验中,SL/SLd 培养中产生的造血细胞数为+/+培养者的10%以下。当+/+培养形成融合贴壁其质层后,进行9、50或200Gy 照射,则见造血停止,观察到10周仍不见恢复。把新鲜SL/SLd骨髓细胞接种到受照9或50Gy+/+贴壁基质层上,
SL / SLd is a hematopoietic microenvironment deficient mouse. Therefore, both in vivo or in vitro, we can see a decrease in the generation of mature hematopoietic cells. The aim of this paper is to study the normal mouse bone marrow matrix correction with and without irradiation, which supports in vitro the ability of SL / SLd bone marrow cells to hematopoietic long term. Cultures inoculated with bone marrow cells of 16 × 10 -6 normal WCB_6F_1 mice (+ / +) or WCB_6F_1-SL / SLd mice were cultured for long term. In 15 separate experiments, the number of hematopoietic cells produced in the SL / SLd culture was less than 10% of the + / + culture. When + / + culture to form a fusion adherent layer of its quality, after 9,50 or 200Gy irradiation, see the hematopoietic stop, observed 10 weeks still no recovery. Fresh SL / SLd bone marrow cells were seeded onto irradiated 9 or 50Gy + / + adherent stromal layers,