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克男和光岛(1958)提出利用多年生野生稻(W_1,Oryza rufipogon Griff)选育细胞质雄性不育[ms-CW]的恢复系,证实可以通过花培将恢复基因(S)从W_1转移到栽培品种中去。以W_1(♀)和栽培品种So-mewake”(?)的杂种F_1代花药,通过培养得到2株具有深染色能力的再生植株,其中1株结实性上表现不育,而另1株(A_1)能育,并且它们的后代(A_2)同样显示出高结实性。以(A_2)植株为父本与携带有W_1胞性而雄性不育的栽培品种“Reimei”测交,结果F_1代具有高的育性,证实再生植株(A_1)具有恢复基因。从F_2和W_1(♀)/Somewake的回交一代(B_1F_1)等常规杂交后代中看出,花粉染色能力及结实性,具有广泛的变异,这不仅归因于细胞质而且也归因于杂种不实性基因。上述结果表明:通过花培途径能有效地利用远缘杂交选育恢复系,尤其是当它们受到杂交不实的干扰时。
Kemang and Kojima (1958) proposed that the restorer line of CMS-MS breeding using the perennial wild rice (W_1, Oryza rufipogon Griff) demonstrated that the restorer gene (S) can be transferred from W_1 Varieties go. Two hybrid F1 progenies with deep dyeing ability were obtained from hybrid F_1 anthers with W_1 (♀) and cultivated So-mewake (?). One of the regenerated plants showed deep staining, ), And their progeny (A_2) also showed high seed set.The cross between the plants (A 2) as male parents and the male sterile cultivars “Reimei” carrying W 1 cells showed that F 1 generation had high Fertility and confirmed that the regenerated plant (A_1) has the restorer gene.From the conventional crossbreeding progenies such as F_2 and W_1 (♀) / Somewake backcross generation (B_1F_1), we can see that pollen dyeing ability and seedability have a wide range of variation, which Not only due to the cytoplasm but also to heterozygous heterozygotes.The above results suggest that the distant hybridization breeding restorer lines can be effectively used by the floral culture pathway, especially when they are misinterpreted by hybridization.