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目的:研究2-Amino-5-phosphonovalerate-pharmacology(AP-V)和荷包牡丹碱对急性低压性低氧大鼠下丘脑内生长抑素(SST)阳性神经元的影响。方法:用免疫组织化学和原位杂交技术检测大鼠下丘脑内SST-IR和SS mRNA阳性神经元数。结果:急性低氧引起下丘脑室周核(PeV)、室旁核(PVN)、弓状核(ARC)SST-IR和SS mRNA阳性神经元数明显增多,该效应可被NMDA受体拮抗剂AP-V(10μg,icv)明显抑制;而GABA_A受体拮抗剂荷包牡丹碱(1.5mg·kg~(-1),ip)则使其增强。结论:这些结果表明下丘脑内的生长抑素参与了急性低压低氧反应,且谷氨酸和GABA分别通过NMDA受体与GABA_A受体影响下丘脑内生长抑素的释放与合成。
AIM: To investigate the effects of 2-Amino-5-phosphonovalerate-pharmacology (AP-V) and bicuculline on somatostatin (SST) -positive neurons in the hypothalamus of rats with acute hypobaric hypoxia. Methods: Immunohistochemistry and in situ hybridization were used to detect the number of SST-IR and SS mRNA positive neurons in rat hypothalamus. Results: Acute hypoxia caused a significant increase in the number of positive neurons of SST-IR and SS mRNA in the nucleus accumbens (PCV), paraventricular nucleus (PVN) and arcuate nucleus (ARC). This effect could be blocked by NMDA receptor antagonist AP-V (10μg, icv) significantly inhibited; and GABA_A receptor antagonist bicuculline (1.5mg · kg -1, ip) make it enhanced. CONCLUSIONS: These results suggest that somatostatin in the hypothalamus is involved in acute hypobaric hypoxia, and glutamate and GABA affect the release and synthesis of somatostatin in the hypothalamus via NMDA and GABA_A receptors, respectively.