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直接凝集实验(direct agglutinationtest,DAT)被认为是一种快速检测内脏利什曼病(VL)的敏感方法,但能否应用于现场尚需评价。本文作者对此方法在现场的可操作性进行了研究。 DAT所用的抗原为杜氏利什曼原虫前鞭毛体。1—2×10~7/ml的前鞭毛体经0.4％胰蛋白酶在37℃清洗60分种后,于2℃经
Direct agglutination test (DAT) is considered as a sensitive method for the rapid detection of visceral leishmaniasis (VL), but its application in the field still needs evaluation. The authors study the operability of this method in the field. The antigen used for DAT is Leishmania donovani promastigotes. 1-2 × 10 ~ 7 / ml Promastigotes by 0.4% trypsin at 37 ℃ for 60 minutes after washing at 2 ℃ by