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以姜黄(Curcuma longa L.)组培苗为试材,在继代培养基中添加不同浓度水杨酸(SA)和硝普钠(SNP,NO供体),研究SA和NO对姜黄生长、次生代谢和姜黄素类化合物含量的影响。试验结果表明:SA和NO均能调节姜黄的生长,促进姜黄次生代谢产物的生成;SA 10μmol·L-1或SNP 200μmol·L-1处理时,姜黄叶片叶绿素含量、类胡萝卜素含量和可溶性糖含量均达到最大值,姜黄生长处于最佳状态。SA和NO均能激活苯丙氨酸解氨酶(PAL)、肉桂酸–4–羟化酶(C4H)和4–香豆酸:辅酶A连接酶(4CL)活性,促进姜黄次生代谢途径中咖啡酸、对香豆酸、阿魏酸和肉桂酸的生成,提高双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素含量;因此,SA 10μmol·L-1或SNP 200μmol·L-1最有利于姜黄生长和次生代谢产物的合成。SA和NO对姜黄次生代谢生理调控具有浓度效应。
Tissue culture seedlings of Curcuma longa L. were used as experimental materials, SA and SNP (NO donor) were added to the subculture medium to study the effects of SA and NO on the growth, Secondary Metabolism and Curcumin Content. The results showed that both SA and NO could regulate the growth of turmeric and promote the formation of secondary metabolites of turmeric. When SA 10 μmol·L-1 or SNP 200 μmol·L-1, the chlorophyll content, carotenoid content and soluble Sugar content reached the maximum, turmeric growth in the best condition. Both SA and NO could activate the activities of PAL, C4H and 4CL and promote the secondary metabolic pathway of turmeric Caffeic acid, p-coumaric acid, ferulic acid and cinnamic acid, increased the content of didemethoxycurcumin, demethoxycurcumin and curcumin; therefore, SA 10 μmol·L-1 or SNP 200 μmol · L-1 is most conducive to the growth of turmeric and secondary metabolites. SA and NO had a concentration effect on the physiological regulation of turmeric secondary metabolism.