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建立反相高效液相色谱荧光检测法测定口服新型咀嚼胶片剂后血浆中右美沙芬浓度的方法。采用Hanbon C18(4.6mm×250mm,5μm)色谱柱,以乙腈-水-磷酸-三乙胺(35∶65∶0.14∶0.10,V/V/V/V)为流动相,在流速0.6mL.min-1,进样量50μL,柱温30℃,荧光激发波长(Ex)和发射波长(Em)分别为280nm和320nm条件下,测定咀嚼胶片剂中右美沙芬的兔血药浓度。药物与杂质分离效果良好、线性范围为1—1000ng.mL-1,相关系数为0.9996。方法回收率和提取回收率分别为110.0%和83.9%。当S/N=3时,右美沙芬最低检出浓度可达1ng.mL-1。本方法准确、灵敏,可满足血药浓度检测和研究药代动力学行为的需要。
To establish a method for the determination of dextromethorphan concentration in plasma following the oral administration of a new chewing gum by fluorescence detection with reversed-phase high performance liquid chromatography. The mobile phase was a Hanbon C18 (4.6 mm × 250 mm, 5 μm) column with acetonitrile-water-phosphoric acid-triethylamine (35:65:14:010, V / V / V / V) at a flow rate of 0.6 mL. min-1, injection volume 50μL, column temperature 30 ℃, dextromethorphan concentration in chewable tablets at 280nm and 320nm, respectively at the fluorescence excitation (Ex) and emission (Em) wavelengths. Drugs and impurities separation effect is good, the linear range of 1-1000ng.mL-1, the correlation coefficient of 0.9996. The recoveries of the method and extraction were 110.0% and 83.9% respectively. When S / N = 3, dextromethorphan minimum detectable concentration of up to 1ng.mL-1. The method is accurate and sensitive, which can meet the need of detecting and studying the pharmacokinetics of plasma concentration.