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目的观察人源性抗日本血吸虫肌球蛋白Fab噬菌体抗体体外杀伤血吸虫童虫作用。方法利用日本血吸虫肌球蛋白(Sj myosin)对人源性抗日本血吸虫Fab噬菌体抗体库进行富集筛选,获得抗Sjmyosin的特异性噬菌体抗体,将该抗体和巨噬细胞或补体与日本血吸虫童虫共培养,测定体外杀伤日本血吸虫童虫作用。结果将抗Sj myosin噬菌体抗体和巨噬细胞及补体与血吸虫童虫共培养时,在培养后24h即出现杀伤作用(童虫死亡率为4.69%),48h童虫死亡率达84.06%,96h童虫死亡率达89.38%。结论人源性抗Sj myosin Fab噬菌体抗体具有体外杀伤日本血吸虫童虫活性。
Objective To observe the killing effect of human anti-Schistosoma japonicum myosin Fab phage antibody against Schistosoma japonicum in vitro. Methods Sj myosin was used to enrich and screen the anti-Sjmyosin Fab phage antibody library, and the anti-Sjmyosin specific phage antibody was obtained. The antibody and macrophages or complement were compared with Schistosoma japonicum Co-culture, determination of in vitro killing Schistosoma japonicum Schistosomiasis. Results The anti-Sj myosin phage antibody, macrophage and complement were co-cultured with Schistosoma japonicum and the killing effect was observed at 24 h (4.69% mortality rate), the mortality rate was 84.06% at 48 h, and the mortality rate was 96.06% Mortality rate of 89.38%. Conclusion The human anti-Sj myosin Fab phage antibody has the activity of killing Schistosoma japonicum in vitro.